› 4dlj

X-ray diffraction
2.6Å resolution

Human p38 MAP kinase in complex with RL163

Released:
DOI: 10.2210/pdb4dlj/pdb
PDB entry 4dlj coloured by chain, front view.
PDB entry 4dlj coloured by chain, side view.
PDB entry 4dlj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Fluorophore labeled kinase detects ligands that bind within the MAPK insert of p38α kinase.
Getlik M, Simard JR, Termathe M, Grütter C, Rabiller M, van Otterlo WA, Rauh D
PLoS One 7 e39713 (2012)
PMID: 22768308

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 14 Chain: A
Molecule details ›
Chain: A
Length: 360 amino acids
Theoretical weight: 41.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16539 (Residues: 2-360; Coverage: 100%)
Gene names: CSBP, CSBP1, CSBP2, CSPB1, MAPK14, MXI2, SAPK2A
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 6RG 2 x 6RG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 65.98Å b: 74.51Å c: 78.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.21 0.314
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Fluorescent biosensors for high throughput screening of protein kinase inhibitors.
Prével et al. (2014)
4 more

5 mentions without citation

Fluorophore labeled kinase detects ligands that bind within the MAPK insert of p38α kinase.
Getlik et al. (2012)
4 more