› 4drb

X-ray diffraction
2.63Å resolution

The crystal structure of FANCM bound MHF complex

Released:
DOI: 10.2210/pdb4drb/pdb
PDB entry 4drb coloured by chain, front view.
PDB entry 4drb coloured by chain, side view.
PDB entry 4drb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the FANCM-MHF complex reveals physical features for functional assembly.
Tao Y, Jin C, Li X, Qi S, Chu L, Niu L, Yao X, Teng M
Nat Commun 3 782 (2012)
PMID: 22510687

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-108753 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Centromere protein S Chains: A, B, D, E, G, H
Molecule details ›
Chains: A, B, D, E, G, H
Length: 120 amino acids
Theoretical weight: 14.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N2Z9 (Residues: 1-114; Coverage: 83%)
Gene names: APITD1, CENPS, FAAP16, MHF1
Sequence domains: CENP-S protein
Structure domains: Histone, subunit A
Fanconi anemia group M protein Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 141 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IYD8 (Residues: 661-800; Coverage: 7%)
Gene names: FANCM, KIAA1596
Sequence domains: FANCM to MHF binding domain
Centromere protein X Chains: J, K, L, M, N, O
Molecule details ›
Chains: J, K, L, M, N, O
Length: 84 amino acids
Theoretical weight: 9.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A8MT69 (Residues: 1-81; Coverage: 100%)
Gene names: CENPX, FAAP10, MHF2, STRA13
Sequence domains: CENP-S associating Centromere protein X
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 111.317Å b: 70.025Å c: 115.751Å
α: 90° β: 91.41° γ: 90°
R-values:
R R work R free
0.222 0.22 0.256
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The Fanconi anemia DNA repair pathway: structural and functional insights into a complex disorder.
Walden et al. (2014)
9 more

4 mentions without citation

Fanconi anemia pathway as a prospective target for cancer intervention.
Liu et al. (2020)
3 more