› 4dtg

X-ray diffraction
1.8Å resolution

Hemostatic effect of a monoclonal antibody mAb 2021 blocking the interaction between FXa and TFPI in a rabbit hemophilia model

Released:
DOI: 10.2210/pdb4dtg/pdb
PDB entry 4dtg coloured by chain, front view.
PDB entry 4dtg coloured by chain, side view.
PDB entry 4dtg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Hemostatic effect of a monoclonal antibody mAb 2021 blocking the interaction between FXa and TFPI in a rabbit hemophilia model.
Hilden I, Lauritzen B, Sørensen BB, Clausen JT, Jespersgaard C, Krogh BO, Bowler AN, Breinholt J, Gruhler A, Svensson LA, Petersen HH, Petersen LC, Balling KW, Hansen L, Hermit MB, Egebjerg T, Friederichsen B, Ezban M, Bjørn SE
Blood 119 5871-8 (2012)
PMID: 22563084

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210487 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Humanized recombinant FAB fragment, FAB 2021, of a murine antibody, light chain Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 23.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Humanized recombinant FAB fragment, FAB 2021, of a murine antibody, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Tissue factor pathway inhibitor Chain: K
Molecule details ›
Chain: K
Length: 66 amino acids
Theoretical weight: 7.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10646 (Residues: 119-178; Coverage: 22%)
Gene names: LACI, TFPI, TFPI1
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

3 bound ligands:
Ligand GOL 10 x GOL
Ligand MES 1 x MES
Ligand PGE 3 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: I222
Unit cell:
a: 65.01Å b: 125.64Å c: 202.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.236
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

45 review citations

Biology of tissue factor pathway inhibitor.
Wood et al. (2014)
44 more

5 mentions without citation

RosettaAntibodyDesign (RAbD): A general framework for computational antibody design.
Adolf-Bryfogle et al. (2018)
4 more