› 4e2i

X-ray diffraction
5Å resolution

The Complex Structure of the SV40 Helicase Large T Antigen and p68 Subunit of DNA Polymerase Alpha-Primase

Released:
DOI: 10.2210/pdb4e2i/pdb
PDB entry 4e2i coloured by chain, front view.
PDB entry 4e2i coloured by chain, side view.
PDB entry 4e2i coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase α-primase.
Zhou B, Arnett DR, Yu X, Brewster A, Sowd GA, Xie CL, Vila S, Gai D, Fanning E, Chen XS
J Biol Chem 287 26854-66 (2012)
PMID: 22700977

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dodecamer
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136542 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Large T antigen Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 362 amino acids
Theoretical weight: 41.81 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: P03070 (Residues: 266-627; Coverage: 51%)
Sequence domains: Polyomavirus large T antigen C-terminus
DNA polymerase alpha subunit B Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, U, W
Molecule details ›
Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, U, W
Length: 78 amino acids
Theoretical weight: 8.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14181 (Residues: 1-78; Coverage: 13%)
Gene name: POLA2

Ligands and Environments

1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P41212
Unit cell:
a: 249.098Å b: 249.098Å c: 387.032Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.305 0.305 0.314
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The E1 proteins.
Bergvall et al. (2013)
7 more

2 mentions without citation

Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase α-primase.
Zhou et al. (2012)
1 more