› 4e2j

X-ray diffraction
2.5Å resolution

X-Ray Crystal Structure of the Ancestral Glucocorticoid Receptor 2 ligand binding domain in complex with mometasone furoate and TIF-2 coactivator fragment

Released:
DOI: 10.2210/pdb4e2j/pdb
PDB entry 4e2j coloured by chain, front view.
PDB entry 4e2j coloured by chain, side view.
PDB entry 4e2j coloured by chain, top view.
Source organisms:
Primary publication:
Deciphering modern glucocorticoid cross-pharmacology using ancestral corticosteroid receptors.
Kohn JA, Deshpande K, Ortlund EA
J Biol Chem 287 16267-75 (2012)
PMID: 22437833

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164417 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ancestral Glucocorticoid Receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 250 amino acids
Theoretical weight: 28.77 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chains: C, E
Molecule details ›
Chains: C, E
Length: 12 amino acids
Theoretical weight: 1.46 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 741-752; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand MOF 2 x MOF
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P61
Unit cell:
a: 104.356Å b: 104.356Å c: 143.925Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Non-genomic effect of glucocorticoids on cardiovascular system.
Lee et al. (2012)
2 more

5 mentions without citation

The Biologist's Guide to the Glucocorticoid Receptor's Structure.
Deploey et al. (2023)
4 more