› 4e52

X-ray diffraction
1.7Å resolution

Crystal structure of Haemophilus Eagan 4A polysaccharide bound human lung surfactant protein D

Released:
DOI: 10.2210/pdb4e52/pdb
PDB entry 4e52 coloured by chain, front view.
PDB entry 4e52 coloured by chain, side view.
PDB entry 4e52 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structure of a Complex of Surfactant Protein D (SP-D) and Haemophilus influenzae Lipopolysaccharide Reveals Shielding of Core Structures in SP-D-Resistant Strains.
Clark HW, Mackay RM, Deadman ME, Hood DW, Madsen J, Moxon ER, Townsend JP, Reid KBM, Ahmed A, Shaw AJ, Greenhough TJ, Shrive AK
Infect Immun 84 1585-1592 (2016)
PMID: 26953329

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Pulmonary surfactant-associated protein D Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 177 amino acids
Theoretical weight: 18.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35247 (Residues: 201-375; Coverage: 49%)
Gene names: COLEC7, PSPD, SFTP4, SFTPD
Sequence domains:
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: GMH, KD5
Carbohydrate polymer
1 bound ligand:
Ligand CA 9 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P21
Unit cell:
a: 55.35Å b: 107.99Å c: 55.65Å
α: 90° β: 92.14° γ: 90°
R-values:
R R work R free
0.192 0.192 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Lipid-Protein and Protein-Protein Interactions in the Pulmonary Surfactant System and Their Role in Lung Homeostasis.
Cañadas et al. (2020)
1 more

4 mentions without citation

Molecular Recognition in C-Type Lectins: The Cases of DC-SIGN, Langerin, MGL, and L-Sectin.
Valverde et al. (2020)
3 more