4e5x

X-ray diffraction
1.95Å resolution

Crystal structure of a complex between the human adenovirus type 2 E3-19K protein and MHC class I molecule HLA-A2/Tax

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-137658 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Protein Tax-1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human T-cell lymphotropic virus type 1 (african isolate)
Expression system: Not provided
UniProt:
  • Canonical: P0C213 (Residues: 11-19; Coverage: 3%)
Gene name: tax
Early E3 18.5 kDa glycoprotein Chains: G, H
Molecule details ›
Chains: G, H
Length: 100 amino acids
Theoretical weight: 11.94 KDa
Source organism: Human adenovirus 6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P68979 (Residues: 18-117; Coverage: 70%)
Sequence domains: Adenovirus GP19K
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: I212121
Unit cell:
a: 113.19Å b: 133.67Å c: 196.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.21 0.241
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided