› 4e61

X-ray diffraction
2.45Å resolution

Crystal structure of the EB1-like motif of Bim1p

Released:
DOI: 10.2210/pdb4e61/pdb
PDB entry 4e61 coloured by chain, front view.
PDB entry 4e61 coloured by chain, side view.
PDB entry 4e61 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Post-translational modifications regulate assembly of early spindle orientation complex in yeast.
Hüls D, Storchova Z, Niessing D
J Biol Chem 287 16238-45 (2012)
PMID: 22461628

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein BIM1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 11.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P40013 (Residues: 182-282; Coverage: 29%)
Gene names: BIM1, YER016W
Sequence domains: EB1-like C-terminal motif
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 28Å b: 42.5Å c: 100.5Å
α: 90° β: 90.3° γ: 90°
R-values:
R R work R free
0.212 0.211 0.241
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Emerging roles of sumoylation in the regulation of actin, microtubules, intermediate filaments, and septins.
Alonso et al. (2015)
1 more

1 mention without citation

Post-translational modifications regulate assembly of early spindle orientation complex in yeast.
Hüls et al. (2012)