› 4ecc

X-ray diffraction
2.2Å resolution

Chimeric GST Containing Inserts of Kininogen Peptides

Released:
DOI: 10.2210/pdb4ecc/pdb
PDB entry 4ecc coloured by chain, front view.
PDB entry 4ecc coloured by chain, side view.
PDB entry 4ecc coloured by chain, top view.
Source organisms:
Primary publication:
Chimeric glutathione S-transferases containing inserts of kininogen peptides: potential novel protein therapeutics.
Bentley AA, Merkulov SM, Peng Y, Rozmarynowycz R, Qi X, Pusztai-Carey M, Merrick WC, Yee VC, McCrae KR, Komar AA
J Biol Chem 287 22142-50 (2012)
PMID: 22577144

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133898 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase class-mu 26 kDa isozyme; Kininogen-1 light chain Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 26.95 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08515 (Residues: 1-49, 50-218; Coverage: 100%)
  • Canonical: P01042 (Residues: 498-510; Coverage: 2%)
Gene names: BDK, KNG, KNG1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 92.52Å b: 92.52Å c: 58.087Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.269
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Kinins and Their Receptors in Infectious Diseases.
Dagnino et al. (2020)
1 more

2 mentions without citation

High Molecular Weight Kininogen: A Review of the Structural Literature.
Ponczek MB. (2021)
1 more