› 4edj

X-ray diffraction
1.9Å resolution

Crystal structure of the GRASP55 GRASP Domain with a phosphomimetic mutation (S189D)

Released:
DOI: 10.2210/pdb4edj/pdb
PDB entry 4edj coloured by chain, front view.
PDB entry 4edj coloured by chain, side view.
PDB entry 4edj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric regulation of GRASP protein-dependent Golgi membrane tethering by mitotic phosphorylation.
Truschel ST, Zhang M, Bachert C, Macbeth MR, Linstedt AD
J Biol Chem 287 19870-5 (2012)
PMID: 22523075

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-190688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Golgi reassembly-stacking protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 22.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9H8Y8 (Residues: 1-208; Coverage: 46%)
Gene names: GOLPH6, GORASP2
Sequence domains: GRASP55/65 PDZ-like domain
Structure domains: Pdz3 Domain

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 64.857Å b: 82.547Å c: 84.059Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.271
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Connecting the cytoskeleton to the endoplasmic reticulum and Golgi.
Gurel et al. (2014)
8 more