› 4edw

X-ray diffraction
2.48Å resolution

Nerve Growth Factor in Complex with Fab from humanized version of mouse mAb 911 (tanezumab)

Released:
DOI: 10.2210/pdb4edw/pdb
PDB entry 4edw coloured by chain, front view.
PDB entry 4edw coloured by chain, side view.
PDB entry 4edw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Generation of a high-fidelity antibody against nerve growth factor using library scanning mutagenesis and validation with structures of the initial and optimized Fab-antigen complexes.
La Porte SL, Eigenbrot C, Ultsch M, Ho WH, Foletti D, Forgie A, Lindquist KC, Shelton DL, Pons J
MAbs 6 1059-68
PMID: 24830649

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208352 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-nerve growth factor Chain: V
Molecule details ›
Chain: V
Length: 120 amino acids
Theoretical weight: 13.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01138 (Residues: 122-241; Coverage: 54%)
Gene names: NGF, NGFB
Sequence domains: Nerve growth factor family
Structure domains: Cystine-knot cytokines
tanezumab Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
tanezumab Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 231 amino acids
Theoretical weight: 24.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2221
Unit cell:
a: 64.7Å b: 92.707Å c: 252.729Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.2 0.249
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

'In-Format' screening of a novel bispecific antibody format reveals significant potency improvements relative to unformatted molecules.
Scott et al. (2017)
1 more

9 mentions without citation

Antibody Specific B-Cell Epitope Predictions: Leveraging Information From Antibody-Antigen Protein Complexes.
Jespersen et al. (2019)
8 more