› 4ef4

X-ray diffraction
2.15Å resolution

Crystal structure of STING CTD complex with c-di-GMP

Released:
DOI: 10.2210/pdb4ef4/pdb
PDB entry 4ef4 coloured by chain, front view.
PDB entry 4ef4 coloured by chain, side view.
PDB entry 4ef4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the STING adaptor protein reveals a hydrophobic dimer interface and mode of cyclic di-GMP binding.
Ouyang S, Song X, Wang Y, Ru H, Shaw N, Jiang Y, Niu F, Zhu Y, Qiu W, Parvatiyar K, Li Y, Zhang R, Cheng G, Liu ZJ
Immunity 36 1073-86 (2012)
PMID: 22579474

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stimulator of interferon genes protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 29.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86WV6 (Residues: 139-379; Coverage: 64%)
Gene names: ERIS, MITA, STING, STING1, TMEM173
Sequence domains: Transmembrane protein 173
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand C2E 1 x C2E
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 61.375Å b: 72.84Å c: 62.44Å
α: 90° β: 97.03° γ: 90°
R-values:
R R work R free
0.211 0.209 0.257
Expression system: Escherichia coli

Quick links

Citations

74 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
73 more

11 mentions without citation

Diversity of Cyclic Di-GMP-Binding Proteins and Mechanisms.
Chou et al. (2016)
10 more