› 4efg

X-ray diffraction
1.58Å resolution

Crystal Structure of the Q108K:K40L:T51V:T53C:Y19W:R58W:T29L Mutant of Cellular Retinol Binding Protein Type II in Complex with All-trans-Retinal at 1.58 Angstrom Resolution

Released:
DOI: 10.2210/pdb4efg/pdb
PDB entry 4efg coloured by chain, front view.
PDB entry 4efg coloured by chain, side view.
PDB entry 4efg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tuning the electronic absorption of protein-embedded all-trans-retinal.
Wang W, Nossoni Z, Berbasova T, Watson CT, Yapici I, Lee KS, Vasileiou C, Geiger JH, Borhan B
Science 338 1340-3 (2012)
PMID: 23224553

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50120 (Residues: 2-134; Coverage: 99%)
Gene names: CRBP2, RBP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand RET 2 x RET
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P1
Unit cell:
a: 30.111Å b: 35.905Å c: 64.726Å
α: 90.93° β: 91.21° γ: 114.13°
R-values:
R R work R free
0.196 0.194 0.242
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.
Ernst et al. (2014)
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4 mentions without citation

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Little MH. (2021)
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