› 4efn

X-ray diffraction
2.3Å resolution

Crystal structure of H-Ras Q61L in complex with GppNHp (state 1)

Released:
DOI: 10.2210/pdb4efn/pdb
PDB entry 4efn coloured by chain, front view.
PDB entry 4efn coloured by chain, side view.
PDB entry 4efn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the state 1 conformations of the GTP-bound H-Ras protein and its oncogenic G12V and Q61L mutants.
Muraoka S, Shima F, Araki M, Inoue T, Yoshimoto A, Ijiri Y, Seki N, Tamura A, Kumasaka T, Yamamoto M, Kataoka T
FEBS Lett 586 1715-8 (2012)
PMID: 22584058

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 19.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: I222
Unit cell:
a: 34.782Å b: 82.006Å c: 121.251Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.237
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Small-molecule modulation of Ras signaling.
Spiegel et al. (2014)
3 more

4 mentions without citation

A structural model of a Ras-Raf signalosome.
Mysore et al. (2021)
3 more