› 4eih

X-ray diffraction
1.2Å resolution

Crystal structure of Arg SH2 domain

Released:
DOI: 10.2210/pdb4eih/pdb
PDB entry 4eih coloured by chain, front view.
PDB entry 4eih coloured by chain, side view.
PDB entry 4eih coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Two amino acid residues confer different binding affinities of Abelson family kinase SRC homology 2 domains for phosphorylated cortactin.
Gifford SM, Liu W, Mader CC, Halo TL, Machida K, Boggon TJ, Koleske AJ
J Biol Chem 289 19704-13 (2014)
PMID: 24891505

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ABL2 Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 12.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P42684 (Residues: 165-273; Coverage: 9%)
Gene names: ABL2, ABLL, ARG
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2221
Unit cell:
a: 56.294Å b: 81.845Å c: 37.686Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.181
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Role of the ABL tyrosine kinases in the epithelial-mesenchymal transition and the metastatic cascade.
Luttman et al. (2021)
1 more

2 mentions without citation

Two amino acid residues confer different binding affinities of Abelson family kinase SRC homology 2 domains for phosphorylated cortactin.
Gifford et al. (2014)
1 more