› 4emt

X-ray diffraction
1.5Å resolution

Crystal Structure of human STING bound to c-di-GMP

Released:
DOI: 10.2210/pdb4emt/pdb
PDB entry 4emt coloured by chain, front view.
PDB entry 4emt coloured by chain, side view.
PDB entry 4emt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of STING bound to cyclic di-GMP reveals the mechanism of cyclic dinucleotide recognition by the immune system.
Shu C, Yi G, Watts T, Kao CC, Li P
Nat Struct Mol Biol 19 722-4 (2012)
PMID: 22728658

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-183240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stimulator of interferon genes protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86WV6 (Residues: 155-341; Coverage: 49%)
Gene names: ERIS, MITA, STING, STING1, TMEM173
Sequence domains: Transmembrane protein 173
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand C2E 1 x C2E
Ligand CA 1 x CA
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2221
Unit cell:
a: 69.797Å b: 78.215Å c: 128.222Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.21
Expression system: Escherichia coli

Quick links

Citations

63 review citations

Innate immune sensing and signaling of cytosolic nucleic acids.
Wu et al. (2014)
62 more

12 mentions without citation

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
11 more