› 4eot

X-ray diffraction
2.86Å resolution

Crystal structure of the MafA homodimer bound to the consensus MARE

Released:
DOI: 10.2210/pdb4eot/pdb
PDB entry 4eot coloured by chain, front view.
PDB entry 4eot coloured by chain, side view.
PDB entry 4eot coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A novel DNA binding mechanism for maf basic region-leucine zipper factors inferred from a MafA-DNA complex structure and binding specificities.
Lu X, Guanga GP, Wan C, Rose RB
Biochemistry 51 9706-17 (2012)
PMID: 23148532

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117430 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor MafA Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 11.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NHW3 (Residues: 226-318; Coverage: 26%)
Gene name: MAFA
Sequence domains: bZIP Maf transcription factor
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
5'-D(*CP*CP*GP*GP*TP*GP*CP*TP*GP*AP*GP*TP*CP*AP*GP*CP*AP*GP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.89 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*CP*CP*CP*TP*GP*CP*TP*GP*AP*CP*TP*CP*AP*GP*CP*AP*CP*CP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.73 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3121
Unit cell:
a: 116.1Å b: 116.1Å c: 85.08Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.26
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

MafA Regulation in β-Cells: From Transcriptional to Post-Translational Mechanisms.
Liang et al. (2022)
7 other articles

4 mentions without citation

The role of natural products in revealing NRF2 function.
Zhang et al. (2020)
3 more