4eoz

X-ray diffraction
2.4Å resolution

Crystal structure of the SPOP BTB domain complexed with the Cul3 N-terminal domain

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-129119 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Speckle-type POZ protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 145 amino acids
Theoretical weight: 16.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O43791 (Residues: 177-319; Coverage: 38%)
Gene name: SPOP
Sequence domains: BTB/POZ domain
Structure domains: Potassium Channel Kv1.1; Chain A
Cullin-3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 364 amino acids
Theoretical weight: 43.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q13618 (Residues: 20-381; Coverage: 47%)
Gene names: CUL3, KIAA0617
Sequence domains: Cullin family
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 213.166Å b: 76.944Å c: 85.834Å
α: 90° β: 108.34° γ: 90°
R-values:
R R work R free
0.214 0.211 0.263
Expression system: Escherichia coli BL21