› 4erk

X-ray diffraction
2.2Å resolution

THE COMPLEX STRUCTURE OF THE MAP KINASE ERK2/OLOMOUCINE

Released:
DOI: 10.2210/pdb4erk/pdb
PDB entry 4erk coloured by chain, front view.
PDB entry 4erk coloured by chain, side view.
PDB entry 4erk coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural basis of inhibitor selectivity in MAP kinases.
Wang Z, Canagarajah BJ, Boehm JC, Kassisà S, Cobb MH, Young PR, Abdel-Meguid S, Adams JL, Goldsmith EJ
Structure 6 1117-28 (1998)
PMID: 9753691

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 42.16 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63086 (Residues: 1-358; Coverage: 100%)
Gene names: Erk2, Mapk, Mapk1, Prkm1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand OLO 1 x OLO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 48.87Å b: 70.26Å c: 59.97Å
α: 90° β: 109.05° γ: 90°
R-values:
R R work R free
0.214 0.214 0.317
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

39 review citations

Fluorine in pharmaceuticals: looking beyond intuition.
Müller et al. (2007)
38 more

6 mentions without citation

Mutations That Confer Drug-Resistance, Oncogenicity and Intrinsic Activity on the ERK MAP Kinases-Current State of the Art.
Smorodinsky-Atias et al. (2020)
5 more