› 4ewi

X-ray diffraction
2.28Å resolution

Crystal structure of the NLRP4 Pyrin domain

Released:
DOI: 10.2210/pdb4ewi/pdb
PDB entry 4ewi coloured by chain, front view.
PDB entry 4ewi coloured by chain, side view.
PDB entry 4ewi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of the NLRP4 pyrin domain.
Eibl C, Grigoriu S, Hessenberger M, Wenger J, Puehringer S, Pinheiro AS, Wagner RN, Proell M, Reed JC, Page R, Diederichs K, Peti W
Biochemistry 51 7330-41 (2012)
PMID: 22928810

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-188630 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NACHT, LRR and PYD domains-containing protein 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 13.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96MN2 (Residues: 1-110; Coverage: 11%)
Gene names: NALP4, NLRP4, PAN2, PYPAF4, RNH2
Sequence domains: PAAD/DAPIN/Pyrin domain
Structure domains: Death Domain, Fas

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3221
Unit cell:
a: 62.107Å b: 62.107Å c: 124.932Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.246
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Assembly and regulation of ASC specks.
Hoss et al. (2017)
14 more

13 mentions without citation

Structure, Activation and Regulation of NLRP3 and AIM2 Inflammasomes.
Sharma et al. (2021)
12 more