› 4ext

X-ray diffraction
1.9Å resolution

Structure of polymerase-interacting domain of human Rev1 in complex with translesional synthesis polymerase zeta

Released:
DOI: 10.2210/pdb4ext/pdb
PDB entry 4ext coloured by chain, front view.
PDB entry 4ext coloured by chain, side view.
PDB entry 4ext coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Liu DN, Ryu KS, Ko JS, Choi BS

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-130061 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA repair protein REV1 Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 11.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBZ9 (Residues: 1156-1251; Coverage: 8%)
Gene names: REV1, REV1L
Sequence domains: DNA repair protein REV1 C-terminal domain
Structure domains: DNA repair protein Rev1, C-terminal domain
DNA polymerase zeta catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 23 amino acids
Theoretical weight: 2.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60673 (Residues: 1873-1895; Coverage: 1%)
Gene names: POLZ, REV3, REV3L
Mitotic spindle assembly checkpoint protein MAD2B Chain: C
Molecule details ›
Chain: C
Length: 204 amino acids
Theoretical weight: 23.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI95 (Residues: 7-209; Coverage: 96%)
Gene names: MAD2B, MAD2L2, REV7
Sequence domains: HORMA domain
Structure domains: HORMA domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 48.699Å b: 71.523Å c: 84.053Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.285
Expression system: Escherichia coli

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