› 4ezm

X-ray diffraction
3.1Å resolution

Crystal structure of the human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23

Released:
DOI: 10.2210/pdb4ezm/pdb
PDB entry 4ezm coloured by chain, front view.
PDB entry 4ezm coloured by chain, side view.
PDB entry 4ezm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of IgE bound to its B-cell receptor CD23 reveals a mechanism of reciprocal allosteric inhibition with high affinity receptor FcεRI.
Dhaliwal B, Yuan D, Pang MO, Henry AJ, Cain K, Oxbrow A, Fabiane SM, Beavil AJ, McDonnell JM, Gould HJ, Sutton BJ
Proc Natl Acad Sci U S A 109 12686-91 (2012)
PMID: 22802656

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134793 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin heavy constant epsilon Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 223 amino acids
Theoretical weight: 24.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01854 (Residues: 209-427; Coverage: 40%)
Gene name: IGHE
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Low affinity immunoglobulin epsilon Fc receptor soluble form Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 143 amino acids
Theoretical weight: 16.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06734 (Residues: 156-298; Coverage: 45%)
Gene names: CD23A, CLEC4J, FCE2, FCER2, IGEBF
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand MAN 2 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 62.89Å b: 110.75Å c: 376.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.283 0.264 0.283
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

19 review citations

Type I and type II Fc receptors regulate innate and adaptive immunity.
Pincetic et al. (2014)
18 more

10 mentions without citation

General mechanism for modulating immunoglobulin effector function.
Sondermann et al. (2013)
9 more