› 4f02

X-ray diffraction
2Å resolution

Crystal structure of the PABP-binding site of eIF4G in complex with RRM1-2 of PABP and poly(A)

Released:
DOI: 10.2210/pdb4f02/pdb
PDB entry 4f02 coloured by chain, front view.
PDB entry 4f02 coloured by chain, side view.
PDB entry 4f02 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Interdomain allostery promotes assembly of the poly(A) mRNA complex with PABP and eIF4G.
Safaee N, Kozlov G, Noronha AM, Xie J, Wilds CJ, Gehring K
Mol Cell 48 375-86 (2012)
PMID: 23041282

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145992 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Polyadenylate-binding protein 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 213 amino acids
Theoretical weight: 23.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11940 (Residues: 1-190; Coverage: 30%)
Gene names: PAB1, PABP, PABP1, PABPC1, PABPC2
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Eukaryotic translation initiation factor 4 gamma 1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 27 amino acids
Theoretical weight: 3.03 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q04637 (Residues: 178-203; Coverage: 2%)
Gene names: EIF4F, EIF4G, EIF4G1, EIF4GI
RNA (5'-R(*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 11 nucleotides
Theoretical weight: 3.58 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P4212
Unit cell:
a: 130.191Å b: 130.191Å c: 86.872Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.204 0.23
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
15 more

14 mentions without citation

eIF4F: a retrospective.
Merrick WC. (2015)
13 more