› 4f3t

X-ray diffraction
2.25Å resolution

Human Argonaute-2 - miR-20a complex

Released:
DOI: 10.2210/pdb4f3t/pdb
PDB entry 4f3t coloured by chain, front view.
PDB entry 4f3t coloured by chain, side view.
PDB entry 4f3t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of human argonaute-2 in complex with miR-20a.
Elkayam E, Kuhn CD, Tocilj A, Haase AD, Greene EM, Hannon GJ, Joshua-Tor L
Cell 150 100-10 (2012)
PMID: 22682761

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193865 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute-2 Chain: A
Molecule details ›
Chain: A
Length: 861 amino acids
Theoretical weight: 97.48 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UKV8 (Residues: 1-859; Coverage: 100%)
Gene names: AGO2, EIF2C2
Sequence domains:
Structure domains:
RNA (5'-R(P*UP*AP*AP*AP*GP*UP*GP*CP*UP*UP*AP*UP*AP*GP*UP*G*CP*AP*GP*G)-3') Chain: R
Molecule details ›
Chain: R
Length: 20 nucleotides
Theoretical weight: 6.45 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand IPH 2 x IPH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 63.427Å b: 107.651Å c: 68.718Å
α: 90° β: 106.69° γ: 90°
R-values:
R R work R free
0.208 0.207 0.254
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

85 review citations

Regulation of microRNA biogenesis.
Ha et al. (2014)
84 more

49 mentions without citation

The evolutionary journey of Argonaute proteins.
Swarts et al. (2014)
48 more