› 4f5b

X-ray diffraction
1.57Å resolution

Triple mutant Src SH2 domain bound to phosphotyrosine

Released:
DOI: 10.2210/pdb4f5b/pdb
PDB entry 4f5b coloured by chain, front view.
PDB entry 4f5b coloured by chain, side view.
PDB entry 4f5b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Superbinder SH2 domains act as antagonists of cell signaling.
Kaneko T, Huang H, Cao X, Li X, Li C, Voss C, Sidhu SS, Li SS
Sci Signal 5 ra68 (2012)
PMID: 23012655

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 12.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12931 (Residues: 144-252; Coverage: 20%)
Gene names: SRC, SRC1
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

1 bound ligand:
Ligand PTR 1 x PTR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P61
Unit cell:
a: 67.6Å b: 67.6Å c: 46.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.198 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Cargo recognition in clathrin-mediated endocytosis.
Traub et al. (2013)
7 more

5 mentions without citation

Chemical biology of protein arginine modifications in epigenetic regulation.
Fuhrmann et al. (2015)
4 more