› 4f5y

X-ray diffraction
2.4Å resolution

Crystal structure of human STING CTD complex with C-di-GMP

Released:
DOI: 10.2210/pdb4f5y/pdb
PDB entry 4f5y coloured by chain, front view.
PDB entry 4f5y coloured by chain, side view.
PDB entry 4f5y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of STING protein reveal basis for recognition of cyclic di-GMP.
Shang G, Zhu D, Li N, Zhang J, Zhu C, Lu D, Liu C, Yu Q, Zhao Y, Xu S, Gu L
Nat Struct Mol Biol 19 725-7 (2012)
PMID: 22728660

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stimulator of interferon genes protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86WV6 (Residues: 149-379; Coverage: 61%)
Gene names: ERIS, MITA, STING, STING1, TMEM173
Sequence domains: Transmembrane protein 173
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand C2E 1 x C2E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 73.145Å b: 81.438Å c: 91.347Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.275
Expression system: Escherichia coli

Quick links

Citations

63 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
62 more

6 mentions without citation

Small molecules targeting the innate immune cGAS‒STING‒TBK1 signaling pathway.
Ding et al. (2020)
5 more