› 4f7t

X-ray diffraction
1.7Å resolution

Crystal Structure of HLA-A*2402 Complexed with a Newly Identified Peptide from 2009 H1N1 PB1 (498-505)

Released:
DOI: 10.2210/pdb4f7t/pdb
PDB entry 4f7t coloured by chain, front view.
PDB entry 4f7t coloured by chain, side view.
PDB entry 4f7t coloured by chain, top view.
Source organisms:
Primary publication:
Cross-allele cytotoxic T lymphocyte responses against 2009 pandemic H1N1 influenza A virus among HLA-A24 and HLA-A3 supertype-positive individuals.
Liu J, Zhang S, Tan S, Yi Y, Wu B, Cao B, Zhu F, Wang C, Wang H, Qi J, Gao GF
J Virol 86 13281-94 (2012)
PMID: 23015716

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137837 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 275 amino acids
Theoretical weight: 31.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
RNA-directed RNA polymerase catalytic subunit Chains: C, F
Molecule details ›
Chains: C, F
Length: 8 amino acids
Theoretical weight: 974 Da
Source organism: Influenza A virus H3N2
Expression system: Not provided
UniProt:
  • Canonical: Q9YXL6 (Residues: 498-505; Coverage: 1%)
Gene name: PB1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 52.838Å b: 66.583Å c: 70.362Å
α: 97.7° β: 101.03° γ: 111.84°
R-values:
R R work R free
0.197 0.196 0.227
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Human T-cell immunity against the emerging and re-emerging viruses.
Zhao et al. (2017)
2 more

4 mentions without citation

Cross-allele cytotoxic T lymphocyte responses against 2009 pandemic H1N1 influenza A virus among HLA-A24 and HLA-A3 supertype-positive individuals.
Liu et al. (2012)
3 more