› 4f9k

X-ray diffraction
2.8Å resolution

Crystal Structure of human cAMP-dependent protein kinase type I-beta regulatory subunit (fragment 11-73), Northeast Structural Genomics Consortium (NESG) Target HR8613A

Released:
DOI: 10.2210/pdb4f9k/pdb
PDB entry 4f9k coloured by chain, front view.
PDB entry 4f9k coloured by chain, side view.
PDB entry 4f9k coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kuzin A, Lew S, Seetharaman J, Maglaqui M, Xiao R, Kohan E, Shastry R, Everett JK, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase type I-beta regulatory subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 95 amino acids
Theoretical weight: 11.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31321 (Residues: 11-73; Coverage: 17%)
Gene name: PRKAR1B
Sequence domains: Regulatory subunit of type II PKA R-subunit
Structure domains: cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 82.27Å b: 50.07Å c: 75.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.254 0.324
Expression system: Escherichia coli BL21(DE3)

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