› 4f9l

X-ray diffraction
3.14Å resolution

Crystal Structure of the Human BTN3A1 Ectodomain in Complex with the 20.1 Single Chain Antibody

Released:
DOI: 10.2210/pdb4f9l/pdb
PDB entry 4f9l coloured by chain, front view.
PDB entry 4f9l coloured by chain, side view.
PDB entry 4f9l coloured by chain, top view.
Source organisms:
Primary publication:
The molecular basis for modulation of human Vγ9Vδ2 T cell responses by CD277/butyrophilin-3 (BTN3A)-specific antibodies.
Palakodeti A, Sandstrom A, Sundaresan L, Harly C, Nedellec S, Olive D, Scotet E, Bonneville M, Adams EJ
J Biol Chem 287 32780-90 (2012)
PMID: 22846996

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-126305 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Butyrophilin subfamily 3 member A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 23.64 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O00481 (Residues: 30-246; Coverage: 45%)
Gene names: BTF5, BTN3A1
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
20.1 anti-BTN3A1 antibody fragment Chains: C, D
Molecule details ›
Chains: C, D
Length: 259 amino acids
Theoretical weight: 27.31 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P4
Unit cell:
a: 165.136Å b: 165.136Å c: 53.807Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.254 0.303
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

36 review citations

Six-of-the-best: unique contributions of γδ T cells to immunology.
Vantourout et al. (2013)
35 more

5 mentions without citation

An Update on the Molecular Basis of Phosphoantigen Recognition by Vγ9Vδ2 T Cells.
Herrmann et al. (2020)
4 more