› 4fao

X-ray diffraction
3.36Å resolution

Specificity and Structure of a high affinity Activin-like 1 (ALK1) signaling complex

Released:
DOI: 10.2210/pdb4fao/pdb
PDB entry 4fao coloured by chain, front view.
PDB entry 4fao coloured by chain, side view.
PDB entry 4fao coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Specificity and structure of a high affinity activin receptor-like kinase 1 (ALK1) signaling complex.
Townson SA, Martinez-Hackert E, Greppi C, Lowden P, Sako D, Liu J, Ucran JA, Liharska K, Underwood KW, Seehra J, Kumar R, Grinberg AV
J Biol Chem 287 27313-25 (2012)
PMID: 22718755

Function and Biology Details

Reaction catalysed: 
ATP + [receptor-protein] = ADP + [receptor-protein] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-153327 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Growth/differentiation factor 2 Chains: A, B, G, H, M, N, S, T, a, b, g, h
Molecule details ›
Chains: A, B, G, H, M, N, S, T, a, b, g, h
Length: 110 amino acids
Theoretical weight: 12.1 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q9UK05 (Residues: 320-429; Coverage: 27%)
Gene names: BMP9, GDF2
Sequence domains: Transforming growth factor beta like domain
Structure domains: Cystine-knot cytokines
Serine/threonine-protein kinase receptor R3 Chains: C, D, I, J, O, P, U, V, c, d, i, j
Molecule details ›
Chains: C, D, I, J, O, P, U, V, c, d, i, j
Length: 106 amino acids
Theoretical weight: 11.59 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P37023 (Residues: 22-118; Coverage: 20%)
Gene names: ACVRL1, ACVRLK1, ALK1
Structure domains: CD59
Activin receptor type-2B Chains: E, F, K, L, Q, R, W, X, e, f, k, l
Molecule details ›
Chains: E, F, K, L, Q, R, W, X, e, f, k, l
Length: 124 amino acids
Theoretical weight: 14.12 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q13705 (Residues: 19-134; Coverage: 24%)
Gene name: ACVR2B
Sequence domains: Activin types I and II receptor domain
Structure domains: CD59

Ligands and Environments

2 bound ligands:
Ligand NAG 24 x NAG
Ligand NA 6 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P3112
Unit cell:
a: 216.453Å b: 216.453Å c: 216.953Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.219 0.261
Expression system: Cricetulus griseus

Quick links

Citations

31 review citations

Structural Biology and Evolution of the TGF-β Family.
Hinck et al. (2016)
30 more

13 mentions without citation

Receptor binding competition: A paradigm for regulating TGF-β family action.
Martinez-Hackert et al. (2021)
12 more