› 4fbn

X-ray diffraction
2.4Å resolution

Insights into structural integration of the PLCgamma regulatory region and mechanism of autoinhibition and activation based on key roles of SH2 domains

Released:
DOI: 10.2210/pdb4fbn/pdb
PDB entry 4fbn coloured by chain, front view.
PDB entry 4fbn coloured by chain, side view.
PDB entry 4fbn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional integration of the PLCγ interaction domains critical for regulatory mechanisms and signaling deregulation.
Bunney TD, Esposito D, Mas-Droux C, Lamber E, Baxendale RW, Martins M, Cole A, Svergun D, Driscoll PC, Katan M
Structure 20 2062-75 (2012)
PMID: 23063561

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148516 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19174 (Residues: 545-790; Coverage: 19%)
Gene names: PLC1, PLCG1
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Unit cell:
a: 53.752Å b: 59.61Å c: 76.923Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.295
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Dysfunction of phospholipase Cγ in immune disorders and cancer.
Koss et al. (2014)
12 more

10 mentions without citation

Netrin-1 up-regulation in inflammatory bowel diseases is required for colorectal cancer progression.
Paradisi et al. (2009)
9 more