› 4fbq

X-ray diffraction
2.5Å resolution

Crystal structure of a covalently fused Nbs1-Mre11 complex with two manganese ions per active site

Released:
DOI: 10.2210/pdb4fbq/pdb
PDB entry 4fbq coloured by chain, front view.
PDB entry 4fbq coloured by chain, side view.
PDB entry 4fbq coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling.
Schiller CB, Lammens K, Guerini I, Coordes B, Feldmann H, Schlauderer F, Möckel C, Schele A, Strässer K, Jackson SP, Hopfner KP
Nat Struct Mol Biol 19 693-700 (2012)
PMID: 22705791

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair and telomere maintenance protein nbs1; Double-strand break repair protein rad32 Chains: A, B
Molecule details ›
Chains: A, B
Length: 472 amino acids
Theoretical weight: 53.35 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O43070 (Residues: 474-531, 542-542; Coverage: 10%)
  • Canonical: Q09683 (Residues: 16-413; Coverage: 61%)
Gene names: SPAC13C5.07, SPBC6B1.09c, nbs1, rad32
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 59.256Å b: 79.032Å c: 222.968Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.257
Expression system: Escherichia coli

Quick links

Citations

27 review citations

DNA damage sensing by the ATM and ATR kinases.
Maréchal et al. (2013)
26 more

4 mentions without citation

Targeting protein-protein interactions in the DNA damage response pathways for cancer chemotherapy.
McPherson et al. (2021)
3 more