› 4fbw

X-ray diffraction
2.2Å resolution

Crystal structure of an unfused Mre11-Nbs1 complex with two manganese ions per active site

Released:
DOI: 10.2210/pdb4fbw/pdb
PDB entry 4fbw coloured by chain, front view.
PDB entry 4fbw coloured by chain, side view.
PDB entry 4fbw coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling.
Schiller CB, Lammens K, Guerini I, Coordes B, Feldmann H, Schlauderer F, Möckel C, Schele A, Strässer K, Jackson SP, Hopfner KP
Nat Struct Mol Biol 19 693-700 (2012)
PMID: 22705791

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-128828 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Double-strand break repair protein rad32 Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 47.38 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09683 (Residues: 7-413; Coverage: 63%)
Gene names: SPAC13C5.07, rad32
Sequence domains:
Structure domains:
DNA repair and telomere maintenance protein nbs1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 59 amino acids
Theoretical weight: 6.98 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O43070 (Residues: 474-531; Coverage: 10%)
Gene names: SPBC6B1.09c, nbs1

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 59.122Å b: 79.955Å c: 220.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.233 0.244
Expression system: Escherichia coli

Quick links

Citations

23 review citations

DNA damage sensing by the ATM and ATR kinases.
Maréchal et al. (2013)
22 more

11 mentions without citation

Repair of double-strand breaks by end joining.
Chiruvella et al. (2013)
10 more