› 4fbx

X-ray diffraction
2.33Å resolution

Complex structure of human protein kinase CK2 catalytic subunit crystallized in the presence of a bisubstrate inhibitor

Released:
DOI: 10.2210/pdb4fbx/pdb
PDB entry 4fbx coloured by chain, front view.
PDB entry 4fbx coloured by chain, side view.
PDB entry 4fbx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A subnanomolar fluorescent probe for protein kinase CK2 interaction studies.
Enkvist E, Viht K, Bischoff N, Vahter J, Saaver S, Raidaru G, Issinger OG, Niefind K, Uri A
Org Biomol Chem 10 8645-53 (2012)
PMID: 23032938

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159384 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Casein kinase II subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 40.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68400 (Residues: 1-335; Coverage: 86%)
Gene names: CK2A1, CSNK2A1
Sequence domains: Protein kinase domain
Structure domains:
bisubstrate inhibitor Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 1.22 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P43212
Unit cell:
a: 72.114Å b: 72.114Å c: 135.056Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.249
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Casein kinases as potential therapeutic targets.
Cozza et al. (2016)
1 more

1 mention without citation

Casein Kinase 2 (CK2)-mediated Phosphorylation of Hsp90β as a Novel Mechanism of Rifampin-induced MDR1 Expression.
Kim et al. (2015)