› 4ff9

X-ray diffraction
2.5Å resolution

Crystal Structure of cysteinylated WT SOD1.

Released:
DOI: 10.2210/pdb4ff9/pdb
PDB entry 4ff9 coloured by chain, front view.
PDB entry 4ff9 coloured by chain, side view.
PDB entry 4ff9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural consequences of cysteinylation of Cu/Zn-superoxide dismutase.
Auclair JR, Brodkin HR, D'Aquino JA, Petsko GA, Ringe D, Agar JN
Biochemistry 52 6145-50 (2013)
PMID: 23919400

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Cu-Zn] Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 15.83 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00441 (Residues: 2-154; Coverage: 99%)
Gene name: SOD1
Sequence domains: Copper/zinc superoxide dismutase (SODC)
Structure domains: Superoxide dismutase, copper/zinc binding domain

Ligands and Environments

3 bound ligands:
Ligand CYS 1 x CYS
Ligand ZN 2 x ZN
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P63
Unit cell:
a: 113.05Å b: 113.05Å c: 70.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.282 0.276 0.335
Expression system: Saccharomyces cerevisiae

Quick links

Citations

6 review citations

Amyloid Oligomers: A Joint Experimental/Computational Perspective on Alzheimer's Disease, Parkinson's Disease, Type II Diabetes, and Amyotrophic Lateral Sclerosis.
Nguyen et al. (2021)
5 more

6 mentions without citation

Structural Properties and Interaction Partners of Familial ALS-Associated SOD1 Mutants.
Huai et al. (2019)
5 more