› 4fk3

X-ray diffraction
2.65Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 33.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15056 (Residues: 444-723; Coverage: 37%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 325 1 x 325
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 51.205Å b: 105.409Å c: 110.473Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.259 0.315
Expression system: Escherichia coli

Quick links

Citations

267 review citations

New driver mutations in non-small-cell lung cancer.
Pao et al. (2011)
266 more

11 mentions without citation

Lessons from Hot Spot Analysis for Fragment-Based Drug Discovery.
Hall et al. (2015)
10 more