› 4fl2

X-ray diffraction
2.19Å resolution

Structural and Biophysical Characterization of the Syk Activation Switch

Released:
DOI: 10.2210/pdb4fl2/pdb
PDB entry 4fl2 coloured by chain, front view.
PDB entry 4fl2 coloured by chain, side view.
PDB entry 4fl2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and biophysical characterization of the Syk activation switch.
Grädler U, Schwarz D, Dresing V, Musil D, Bomke J, Frech M, Greiner H, Jäkel S, Rysiok T, Müller-Pompalla D, Wegener A
J Mol Biol 425 309-33 (2013)
PMID: 23154170

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase SYK Chain: A
Molecule details ›
Chain: A
Length: 636 amino acids
Theoretical weight: 72.22 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P43405 (Residues: 1-635; Coverage: 100%)
Gene name: SYK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 62.562Å b: 70.722Å c: 76.369Å
α: 90° β: 103.84° γ: 90°
R-values:
R R work R free
0.184 0.184 0.228
Expression system: Trichoplusia ni

Quick links

Citations

9 review citations

Getting Syk: spleen tyrosine kinase as a therapeutic target.
Geahlen RL. (2014)
8 more

12 mentions without citation

Structural basis for activation of ZAP-70 by phosphorylation of the SH2-kinase linker.
Yan et al. (2013)
11 more