› 4fl5

X-ray diffraction
1.9Å resolution

Crystal structure of human 14-3-3 sigma in complex with a Tau-protein peptide surrounding pS214

Released:
DOI: 10.2210/pdb4fl5/pdb
PDB entry 4fl5 coloured by chain, front view.
PDB entry 4fl5 coloured by chain, side view.
PDB entry 4fl5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Involvement of 14-3-3 in tubulin instability and impaired axon development is mediated by Tau.
Joo Y, Schumacher B, Landrieu I, Bartel M, Smet-Nocca C, Jang A, Choi HS, Jeon NL, Chang KA, Kim HS, Ottmann C, Suh YH
FASEB J 29 4133-44 (2015)
PMID: 26103986

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145399 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 26.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Microtubule-associated protein tau Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 10 amino acids
Theoretical weight: 1.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10636 (Residues: 527-536; Coverage: 1%)
Gene names: MAPT, MAPTL, MTBT1, TAU

Ligands and Environments

4 bound ligands:
Ligand CL 2 x CL
Ligand MG 4 x MG
Ligand CA 6 x CA
Ligand GOL 3 x GOL
2 modified residues:
Ligand CSO 2 x CSO
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 62.69Å b: 70.08Å c: 128.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.156 0.212
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Much More Than a Cytoskeletal Protein: Physiological and Pathological Functions of the Non-microtubule Binding Region of Tau.
Brandt et al. (2020)
9 more

6 mentions without citation

Modulators of 14-3-3 Protein-Protein Interactions.
Stevers et al. (2018)
5 more