› 4fm9

X-ray diffraction
2.9Å resolution

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117506 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA topoisomerase 2-alpha Chain: A
Molecule details ›
Chain: A
Length: 763 amino acids
Theoretical weight: 88.16 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P11388 (Residues: 431-1193; Coverage: 50%)
Gene names: TOP2, TOP2A
Sequence domains:
Structure domains:
DNA (5'-D(P*GP*AP*GP*GP*AP*TP*GP*AP*CP*GP*AP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4.08 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*GP*CP*GP*CP*AP*TP*CP*GP*TP*CP*AP*TP*CP*CP*TP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 17 nucleotides
Theoretical weight: 5.1 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 85.918Å b: 215.081Å c: 128.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.27
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided

Quick links

Citations

29 review citations

New mechanistic and functional insights into DNA topoisomerases.
Chen et al. (2013)
28 more

35 mentions without citation

Copaifera of the Neotropics: A Review of the Phytochemistry and Pharmacology.
da Trindade et al. (2018)
34 more