› 4fvq

X-ray diffraction
1.75Å resolution

Crystal structure of the Jak2 pseudokinase domain (Mg-ATP-bound form)

Released:
DOI: 10.2210/pdb4fvq/pdb
PDB entry 4fvq coloured by chain, front view.
PDB entry 4fvq coloured by chain, side view.
PDB entry 4fvq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the JAK2 pseudokinase domain and the pathogenic mutant V617F.
Bandaranayake RM, Ungureanu D, Shan Y, Shaw DE, Silvennoinen O, Hubbard SR
Nat Struct Mol Biol 19 754-9 (2012)
PMID: 22820988

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase JAK2 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 33.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60674 (Residues: 536-812; Coverage: 25%)
Gene name: JAK2
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 1 x MG
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 44.632Å b: 57.548Å c: 60.967Å
α: 90° β: 110.85° γ: 90°
R-values:
R R work R free
0.176 0.175 0.199
Expression system: Spodoptera frugiperda

Quick links

Citations

55 review citations

The JAK-STAT pathway: impact on human disease and therapeutic intervention.
O'Shea et al. (2015)
54 more

18 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
17 more