› 4fxw

X-ray diffraction
2.29Å resolution

Structure of phosphorylated SF1 complex with U2AF65-UHM domain

Released:
DOI: 10.2210/pdb4fxw/pdb
PDB entry 4fxw coloured by chain, front view.
PDB entry 4fxw coloured by chain, side view.
PDB entry 4fxw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex.
Wang W, Maucuer A, Gupta A, Manceau V, Thickman KR, Bauer WJ, Kennedy SD, Wedekind JE, Green MR, Kielkopf CL
Structure 21 197-208 (2013)
PMID: 23273425

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150720 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 106 amino acids
Theoretical weight: 12.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26368 (Residues: 375-475; Coverage: 21%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Splicing factor 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 124 amino acids
Theoretical weight: 14.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15637 (Residues: 14-132; Coverage: 19%)
Gene names: SF1, ZFM1, ZNF162
Sequence domains: Splicing factor 1 helix-hairpin domain

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
2 modified residues:
Ligand MSE 12 x MSE
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2221
Unit cell:
a: 76.8Å b: 116.1Å c: 130Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.248 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Deciphering the protein-RNA recognition code: combining large-scale quantitative methods with structural biology.
Hennig et al. (2015)
4 more

12 mentions without citation

Structural studies of the spliceosome: zooming into the heart of the machine.
Galej et al. (2014)
11 more