› 4ga8

X-ray diffraction
1.94Å resolution

Human AKR1B10 mutant V301L complexed with NADP+ and sorbinil

Released:
DOI: 10.2210/pdb4ga8/pdb
PDB entry 4ga8 coloured by chain, front view.
PDB entry 4ga8 coloured by chain, side view.
PDB entry 4ga8 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Cousido-Siah A, Ruiz Figueras FX, Mitschler A, Podjarny A

Function and Biology Details

Reactions catalysed: 
All-trans-retinol + NADP(+) = all-trans-retinal + NADPH
Allyl alcohol + NADP(+) = acrolein + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129914 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldo-keto reductase family 1 member B10 Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 36.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60218 (Residues: 1-316; Coverage: 100%)
Gene names: AKR1B10, AKR1B11
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
1 bound ligand:
Ligand SBI 1 x SBI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P61
Unit cell:
a: 89.3Å b: 89.3Å c: 78.155Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.151 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links