Function and Biology Details
Reaction catalysed:
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184887 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DCN1-like protein 3
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Cullin binding
Structure domains:
Length: 221 amino acids
Theoretical weight: 25.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q8IWE4 (Residues: 86-304; Coverage: 72%)
Sequence domains: Cullin binding
Structure domains:
NEDD8-conjugating enzyme UBE2F
Molecule details ›
Chains: F, G
Length: 25 amino acids
Theoretical weight: 2.65 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
Length: 25 amino acids
Theoretical weight: 2.65 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
- Canonical: Q969M7 (Residues: 1-25; Coverage: 14%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ALS BEAMLINE 8.2.2
Spacegroup:
P21
Expression systems:
- Escherichia coli
- Not provided
