› 4gbv

X-ray diffraction
2.9Å resolution

Crystal Structure of AMP complexes of Porcine Liver Fructose-1,6-bisphosphatase Mutant A54L with 1,2-ethanediol as Cryo-protectant

Released:
DOI: 10.2210/pdb4gbv/pdb
PDB entry 4gbv coloured by chain, front view.
PDB entry 4gbv coloured by chain, side view.
PDB entry 4gbv coloured by chain, top view.
Source organism: Sus scrofa
Entry authors: Honzatko RB, Gao Y

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase 1 Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 36.53 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00636 (Residues: 2-336; Coverage: 99%)
Gene names: FBP, FBP1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand F6P 1 x F6P
Ligand PO4 1 x PO4
Ligand MN 2 x MN
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Cu FINE FOCUS
Spacegroup: I222
Unit cell:
a: 53.69Å b: 82.506Å c: 166.284Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.243
Expression system: Escherichia coli

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