› 4gh7

X-ray diffraction
2.6Å resolution

Crystal structure of Anticalin N7A in complex with oncofetal fibronectin fragment Fn7B8

Released:
DOI: 10.2210/pdb4gh7/pdb
PDB entry 4gh7 coloured by chain, front view.
PDB entry 4gh7 coloured by chain, side view.
PDB entry 4gh7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Combinatorial design of an Anticalin directed against the extra-domain b for the specific targeting of oncofetal fibronectin.
Gebauer M, Schiefner A, Matschiner G, Skerra A
J Mol Biol 425 780-802 (2013)
PMID: 23238252

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136137 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neutrophil gelatinase-associated lipocalin Chains: A, C
Molecule details ›
Chains: A, C
Length: 188 amino acids
Theoretical weight: 21.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P80188 (Residues: 21-198; Coverage: 100%)
Gene names: HNL, LCN2, NGAL
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin
Ugl-Y3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 285 amino acids
Theoretical weight: 30.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02751 (Residues: 1173-1448; Coverage: 11%)
Gene names: FN, FN1
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 107.126Å b: 107.126Å c: 233.156Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.259
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Lipocalin 2: An Emerging Player in Iron Homeostasis and Inflammation.
Xiao et al. (2017)
11 more

6 mentions without citation

Beyond Antibodies as Binding Partners: The Role of Antibody Mimetics in Bioanalysis.
Yu et al. (2017)
5 more