› 4gi0

X-ray diffraction
2.27Å resolution

Crystal structure of CD23 lectin domain mutant E249A

Released:
DOI: 10.2210/pdb4gi0/pdb
PDB entry 4gi0 coloured by chain, front view.
PDB entry 4gi0 coloured by chain, side view.
PDB entry 4gi0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ca2+-dependent structural changes in the B-cell receptor CD23 increase its affinity for human immunoglobulin E.
Yuan D, Keeble AH, Hibbert RG, Fabiane S, Gould HJ, McDonnell JM, Beavil AJ, Sutton BJ, Dhaliwal B
J Biol Chem 288 21667-77 (2013)
PMID: 23775083

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139192 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Low affinity immunoglobulin epsilon Fc receptor soluble form Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 143 amino acids
Theoretical weight: 16.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06734 (Residues: 156-298; Coverage: 45%)
Gene names: CD23A, CLEC4J, FCE2, FCER2, IGEBF
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P4122
Unit cell:
a: 63.32Å b: 63.32Å c: 261.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.251
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structure and dynamics of IgE-receptor interactions: FcεRI and CD23/FcεRII.
Sutton et al. (2015)
6 more

1 mention without citation

Ca2+-dependent structural changes in the B-cell receptor CD23 increase its affinity for human immunoglobulin E.
Yuan et al. (2013)