› 4gl2

X-ray diffraction
3.56Å resolution

Structural Basis for dsRNA duplex backbone recognition by MDA5

Released:
DOI: 10.2210/pdb4gl2/pdb
PDB entry 4gl2 coloured by chain, front view.
PDB entry 4gl2 coloured by chain, side view.
PDB entry 4gl2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for dsRNA recognition, filament formation, and antiviral signal activation by MDA5.
Wu B, Peisley A, Richards C, Yao H, Zeng X, Lin C, Chu F, Walz T, Hur S
Cell 152 276-89 (2013)
PMID: 23273991

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189848 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Interferon-induced helicase C domain-containing protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 699 amino acids
Theoretical weight: 80.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BYX4 (Residues: 306-1017; Coverage: 68%)
Gene names: IFIH1, MDA5, RH116
Sequence domains:
Structure domains:
RNA (5'-R(*AP*UP*CP*CP*GP*CP*GP*GP*CP*CP*CP*U)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 12 nucleotides
Theoretical weight: 3.76 KDa
Source organism: Homo sapiens
Expression system: Not provided
RNA (5'-R(P*AP*GP*GP*GP*CP*CP*GP*CP*GP*GP*AP*U)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 12 nucleotides
Theoretical weight: 3.91 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 71.112Å b: 154.748Å c: 185.006Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.278 0.276 0.32
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

152 review citations

Classification of intrinsically disordered regions and proteins.
van der Lee et al. (2014)
151 more

27 mentions without citation

Double-Stranded RNA Sensors and Modulators in Innate Immunity.
Hur S. (2019)
26 more