› 4gnk

X-ray diffraction
4Å resolution

Crystal structure of Galphaq in complex with full-length human PLCbeta3

Released:
DOI: 10.2210/pdb4gnk/pdb
PDB entry 4gnk coloured by chain, front view.
PDB entry 4gnk coloured by chain, side view.
PDB entry 4gnk coloured by chain, top view.
Source organisms:
Primary publication:
Full-length Gα(q)-phospholipase C-β3 structure reveals interfaces of the C-terminal coiled-coil domain.
Lyon AM, Dutta S, Boguth CA, Skiniotis G, Tesmer JJ
Nat Struct Mol Biol 20 355-62 (2013)
PMID: 23377541

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-149200 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Guanine nucleotide-binding protein G(q) subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 353 amino acids
Theoretical weight: 41.54 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P21279 (Residues: 7-359; Coverage: 98%)
Gene name: Gnaq
Sequence domains: G-protein alpha subunit
Structure domains:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 1235 amino acids
Theoretical weight: 139.32 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q01970 (Residues: 10-1234; Coverage: 99%)
Gene name: PLCB3
Sequence domains:
Structure domains:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Chain: E
Molecule details ›
Chain: E
Length: 259 amino acids
Theoretical weight: 30.35 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q01970 (Residues: 934-1192; Coverage: 21%)
Gene name: PLCB3
Sequence domains: PLC-beta C terminal
Structure domains: Phospholipase C beta, distal C-terminal domain

Ligands and Environments

4 bound ligands:
Ligand GDP 2 x GDP
Ligand ALF 2 x ALF
Ligand MG 2 x MG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D, APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 91.423Å b: 188.845Å c: 293.857Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.255
Expression system: Trichoplusia ni

Quick links

Citations

8 review citations

Phosphatidylinositol(4,5)bisphosphate: diverse functions at the plasma membrane.
Katan et al. (2020)
7 more

20 mentions without citation

Structural insights into phospholipase C-β function.
Lyon et al. (2013)
19 more