› 4goy

X-ray diffraction
2.3Å resolution

The crystal structure of human fascin 1 K41A mutant

Released:
DOI: 10.2210/pdb4goy/pdb
PDB entry 4goy coloured by chain, front view.
PDB entry 4goy coloured by chain, side view.
PDB entry 4goy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanism of fascin function in filopodial formation.
Yang S, Huang FK, Huang J, Chen S, Jakoncic J, Leo-Macias A, Diaz-Avalos R, Chen L, Zhang JJ, Huang XY
J Biol Chem 288 274-84 (2013)
PMID: 23184945

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-172542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fascin Chains: A, B
Molecule details ›
Chains: A, B
Length: 493 amino acids
Theoretical weight: 54.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16658 (Residues: 1-493; Coverage: 100%)
Gene names: FAN1, FSCN1, HSN, SNL
Sequence domains: Fascin domain
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

4 bound ligands:
Ligand BR 8 x BR
Ligand CL 10 x CL
Ligand GOL 6 x GOL
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 161.138Å b: 70.595Å c: 110.979Å
α: 90° β: 130.63° γ: 90°
R-values:
R R work R free
0.204 0.201 0.245
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Dynamic aberrant NF-κB spurs tumorigenesis: a new model encompassing the microenvironment.
Vlahopoulos et al. (2015)
10 more

1 mention without citation

Molecular mechanism of fascin function in filopodial formation.
Yang et al. (2013)