› 4grg

X-ray diffraction
4.24Å resolution

Crystal structure of IgE complexed with E2_79, an anti-IgE inhibitor

Released:
DOI: 10.2210/pdb4grg/pdb
PDB entry 4grg coloured by chain, front view.
PDB entry 4grg coloured by chain, side view.
PDB entry 4grg coloured by chain, top view.
Source organisms:
Primary publication:
Accelerated disassembly of IgE-receptor complexes by a disruptive macromolecular inhibitor.
Kim B, Eggel A, Tarchevskaya SS, Vogel M, Prinz H, Jardetzky TS
Nature 491 613-7 (2012)
PMID: 23103871

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134796 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ANTI-IGE INHIBITOR E2_79 Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 14.65 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Immunoglobulin heavy constant epsilon Chains: C, D
Molecule details ›
Chains: C, D
Length: 230 amino acids
Theoretical weight: 25.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01854 (Residues: 210-427; Coverage: 40%)
Gene name: IGHE
Sequence domains: Immunoglobulin C1-set domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P32
Unit cell:
a: 71.315Å b: 71.315Å c: 178.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.273 0.27 0.338
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

31 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
30 more

9 mentions without citation

Accelerated disassembly of IgE-receptor complexes by a disruptive macromolecular inhibitor.
Kim et al. (2012)
8 more